The use of Anti-Müllerian Hormone (AMH) in canine breeding programs could be a game-changer. In a recent study, researchers established a normal reference interval for AMH in breeding bitches and found a positive correlation between AMH concentration and litter size. This suggests that AMH testing could be used to predict litter size or give an idea of a bitch's breeding potential. By identifying bitches with higher AMH levels, veterinary clinics could optimize breeding potential and minimize breeding-related risks. However, AMH testing is not without its limitations, and more research is needed to fully understand its role in predicting litter size and fecundity. Nonetheless, the discovery of AMH is an exciting development in the field of canine reproduction and could lead to the development of more accurate and precise testing methods.